JiscMail Logo
Email discussion lists for the UK Education and Research communities

Help for CCP4BB Archives


CCP4BB Archives

CCP4BB Archives


CCP4BB@JISCMAIL.AC.UK


View:

Message:

[

First

|

Previous

|

Next

|

Last

]

By Topic:

[

First

|

Previous

|

Next

|

Last

]

By Author:

[

First

|

Previous

|

Next

|

Last

]

Font:

Proportional Font

LISTSERV Archives

LISTSERV Archives

CCP4BB Home

CCP4BB Home

CCP4BB  April 2009

CCP4BB April 2009

Options

Subscribe or Unsubscribe

Subscribe or Unsubscribe

Log In

Log In

Get Password

Get Password

Subject:

Postdoctoral position available

From:

Stewart Turley <[log in to unmask]>

Reply-To:

Stewart Turley <[log in to unmask]>

Date:

Thu, 2 Apr 2009 13:33:01 -0700

Content-Type:

TEXT/PLAIN

Parts/Attachments:

Parts/Attachments

TEXT/PLAIN (99 lines)

Postdoctoral Position available in the Laboratory of Wim Hol
Department of Biochemistry, School of Medicine,
University of Washington, Seattle, USA

Structural Biology and structure-based inhibitor design of the invasion
machinery of the malaria parasite.



JOB DESCRIPTION:

 	This project aims to unravel the structure of the invasion machinery of the
malaria parasite and to design inhibitors to interfere with the proper
functioning of the machinery. Such inhibitors might be the foundation for new
anti-malarials.

 	The malaria parasite invades two types of cells when in the human host:
hepatocytes and erythrocytes. This invasion is a fascinating and very complex
process, but some features are shared in the invasion of these two different
cell types. These include aldolase (yes, the glycolytic enzyme is moonlighting
here...) interacting with an actin filament, a specific myosin motor interacting
with the actin, a myosin-tail interacting protein (MTIP), and a number of
so-called GAP proteins. We have elucidated several structures of interacting
proteins in this system. There are also exciting invasion-blocking compounds
which we try to co-crystallize with their target protein.

 	The aims for the project in the next few years are; (i) to obtain additional
structural information about additional proteins and multi-protein complexes of
the machinery; and, (ii)
to assist in structure-based design of invasion inhibitors.

 	The project is an interdisciplinary collaboration with molecular modeling,
chemical synthesis, parasitolology and biochemical groups, in the Biomolecular
Structure Center of the University of Washington, Seattle, and Drexel
University, Philadelphia.

 	The successful candidate will have the opportunity to: (i) carry out protein
expression and protein chemistry studies to obtain insight into protein-protein
interactions involving the invasion machinery of the malaria parasite; (ii)
determine high resolution crystal structures of individual proteins as well as
multi-protein sub-complexes of the machinery; (iii) solve crystal structures of
invasion proteins in complex with small molecule inhibitors. There will also be
opportunities to carry out biophysical binding assays. Several expression
systems are already available for preparing soluble proteins from the invasion
machinery.

 	For further information regarding our studies on the invasion machinery see:

Bosch, J., Turley, S., Daly, T. M., Bogh, S. M., Villasimil, M. L., Roach, C.,
Zhou, N., Morrisey, J. M., Vaidya, A. B., Bergman, L. W. & Hol, W. G. J. (2006).
Structure of the Plasmodium MTIP-MyoA complex, a key component of the malaria
parasite invasion motor. Proc. Natl. Acad. Sci. USA 103, 4852-4857.

Bosch, J., Buscaglia, C. A., Krumm, B. E., Ingason, B., Lucas, R., Roach, C.,
Cardozo, T., Nussenzweig, V. & Hol, W. G. (2007). Aldolase provides an unusual
binding site for TRAP in the invasion machinery of the malaria parasite. Proc.
Natl. Acad. Sci. USA, 104, 7015-8020.
Bosch, J., Turley, S., Roach, C., Daly, T. M., Bergman, L. W. & Hol, W. G.
(2007) The Closed MTIP-MyosinA-tail Complex from the Malaria Parasite Invasion
Machinery. J Mol Biol. 372, 77-88.

And for information regarding the research in our lab see the websites:
http://www.bmsc.washington.edu/WimHol/
http://depts.washington.edu/biowww/faculty/hol.html

JOB REQUIREMENTS:  - Experience with:
 	- protein expression and purification methods
 	- characterization of purified soluble proteins
 	- protein crystallization
- At least three years of experience with protein structure determination
methods, including:
 	X-ray diffraction data collection and data processing; experimental and
molecular replacement phasing methods; density modification; crystallographic
refinement.
- Excellent interpersonal skills to function optimally in the malaria invasion
project team and to 	cooperate with collaborators in other groups and
institutions.


Experience in one or more of the following fields would be a plus:
- Molecular biology for protein overexpression
- Purification and characterization of multi-protein complexes
- Biophysical binding assays
- Molecular modeling for inhibitor design.


START DATE:		Immediately

INSTITUTION: 		Department of Biochemistry
 			Biomolecular Structure Center
 			School of Medicine
 			Box 357742
 			University of Washington
 			Seattle, WA, 98195  USA

Please send your CV, including a description of your experience, a list of
publications, and names and email addresses of three references able to assess
your scientific experience and capabilities to: [log in to unmask]

Top of Message | Previous Page | Permalink

JiscMail Tools


RSS Feeds and Sharing


Advanced Options


Archives

March 2024
February 2024
January 2024
December 2023
November 2023
October 2023
September 2023
August 2023
July 2023
June 2023
May 2023
April 2023
March 2023
February 2023
January 2023
December 2022
November 2022
October 2022
September 2022
August 2022
July 2022
June 2022
May 2022
April 2022
March 2022
February 2022
January 2022
December 2021
November 2021
October 2021
September 2021
August 2021
July 2021
June 2021
May 2021
April 2021
March 2021
February 2021
January 2021
December 2020
November 2020
October 2020
September 2020
August 2020
July 2020
June 2020
May 2020
April 2020
March 2020
February 2020
January 2020
December 2019
November 2019
October 2019
September 2019
August 2019
July 2019
June 2019
May 2019
April 2019
March 2019
February 2019
January 2019
December 2018
November 2018
October 2018
September 2018
August 2018
July 2018
June 2018
May 2018
April 2018
March 2018
February 2018
January 2018
December 2017
November 2017
October 2017
September 2017
August 2017
July 2017
June 2017
May 2017
April 2017
March 2017
February 2017
January 2017
December 2016
November 2016
October 2016
September 2016
August 2016
July 2016
June 2016
May 2016
April 2016
March 2016
February 2016
January 2016
December 2015
November 2015
October 2015
September 2015
August 2015
July 2015
June 2015
May 2015
April 2015
March 2015
February 2015
January 2015
December 2014
November 2014
October 2014
September 2014
August 2014
July 2014
June 2014
May 2014
April 2014
March 2014
February 2014
January 2014
December 2013
November 2013
October 2013
September 2013
August 2013
July 2013
June 2013
May 2013
April 2013
March 2013
February 2013
January 2013
December 2012
November 2012
October 2012
September 2012
August 2012
July 2012
June 2012
May 2012
April 2012
March 2012
February 2012
January 2012
December 2011
November 2011
October 2011
September 2011
August 2011
July 2011
June 2011
May 2011
April 2011
March 2011
February 2011
January 2011
December 2010
November 2010
October 2010
September 2010
August 2010
July 2010
June 2010
May 2010
April 2010
March 2010
February 2010
January 2010
December 2009
November 2009
October 2009
September 2009
August 2009
July 2009
June 2009
May 2009
April 2009
March 2009
February 2009
January 2009
December 2008
November 2008
October 2008
September 2008
August 2008
July 2008
June 2008
May 2008
April 2008
March 2008
February 2008
January 2008
December 2007
November 2007
October 2007
September 2007
August 2007
July 2007
June 2007
May 2007
April 2007
March 2007
February 2007
January 2007


JiscMail is a Jisc service.

View our service policies at https://www.jiscmail.ac.uk/policyandsecurity/ and Jisc's privacy policy at https://www.jisc.ac.uk/website/privacy-notice

For help and support help@jisc.ac.uk

Secured by F-Secure Anti-Virus CataList Email List Search Powered by the LISTSERV Email List Manager