Look at the moment plots after scalepack2mtz; if these are normal it
seems very unlikely you have twinning..
Eleanor
Kumar wrote:
> Hi,
>
> I am trying to solve a protein-RNA complex structure - space group is either
> P4(1)22 or P4(3)22. Even though data seems to have enough Se-anomalous
> signal, we are unable to get any solution. I have tried even lower symmetry
> such as P4(1), P4(3), P4, P222(1), C222(1), and P2(1).
> Now I am worried if my data is twinned. Cumulative intensity distribution
> (using scalepack2mtz) looks normal in all the space groups. Data analysis
> using phenix.xtriage indicates presence of perfect twinning or higher
> symmetry when analyzed in space group P4(1) or P4(3). As I have read, when
> twin axis is parallel to one of the symmetry axis, data can show
> artificially higher symmetry and cumulative intensity distribution in such
> cases shows normal profile. I can work on improving anomalous signal if it
> is not good enough but before that I want to be sure if data is not twinned.
> Now how do I make sure that my data is fine and not twinned.
>
> I need your suggestions.
>
> Thank you
> Kuma
>
oo
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