Hi
To be able to pick negative peaks go to M;Peaks:Peak finding and then toggle the "Extrema to search for" to "positive and negative", it is set to "positive only" by default.
Cheers
Ben
Ben Goult, Ph.D.
University of Leicester
Dept Biochemistry, Room 1/40
Henry Wellcome Building
Lancaster Road
Leicester, UK
LE1 9HN
Tel : +44 (0) 116-229-7110
Fax: +44 (0) 116-229-7084
________________________________________
From: CcpNmr software mailing list [[log in to unmask]] On Behalf Of shi jiahai [[log in to unmask]]
Sent: 25 March 2009 04:26
To: [log in to unmask]
Subject: Re: can not pick up the negative peak on HNCACB spectra
Dear Patrick
Thank you very much for your suggestion.
I am using the version 2.0. The negative peaks can be identified from the spectra clearly. In the detail setting of contour, the negative peak is enable. And the auto levels is applied in the Negative levels. I attached a snapshot of the setting. I can pick the positive one but not the negative one.
Any help would be greatly appreciated!
Thank you very much!
Best Regards,
SHi jiahai
2009/3/25 Patrick van der Wel <[log in to unmask]<mailto:[log in to unmask]>>
Hi,
Do you not see the negative peaks? It might be that the program is set
up to only display the positive peaks by default. This is the case in at
least the older version (1.015). If you click on the contours tab in the
spectrum window, and then select the 'Detailed settings' button, you
might see that the Negative contours are not enabled/selected (in the
Auto contours section).
Patrick
On 3/24/2009 11:11 PM, shi jiahai wrote:
> Dear all,
>
> I am new to CCPNMR. I would like to ask one simple question.
>
> I am doing the sequential assignment from HNCACB/CBCACONH 3D
> experiemnt. The experiment types are set to ( H[N[(CA|ca[Cali]}]] and
> H[N[co[{CA|caC}]]] ). However I can't pick the negative peaks on the
> HNCACB spectra. The positive peaks are normal.
>
> Any help would be greatly appreciated!
>
> Thank you very much!
> Best Regards,
> SHi jiahai
--
Best Regards,
Shi Jiahai
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