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CCP4BB Home

CCP4BB  March 2009

CCP4BB March 2009

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Subject:

Re: images

From:

Liz Potterton <[log in to unmask]>

Reply-To:

Liz Potterton <[log in to unmask]>

Date:

Wed, 18 Mar 2009 17:16:38 +0000

Content-Type:

text/plain

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Parts/Attachments

text/plain (125 lines)

Dear CCP4BB,

For further  research of a particular structure wouldn't hints on
expressing, purifying and crystalizing the protein often be more
useful than images or amplitudes?

Liz


On 18 Mar 2009, at 17:00, Ethan Merritt wrote:

> On Wednesday 18 March 2009 09:41:59 Garib Murshudov wrote:
>> Dear all
>>
>> Before going into and trying to find a technical solution to the
>> problem it would be good if decide if we need images. As far as I
>> know
>> if we face with a problem to solve and we know that it is necessary
>> to
>> solve then we find technical solution to the problem (either from
>> other fields or we find our own solution with some elements of
>> reinvention of new MX wheels).
>>
>> Do we need images to store? What kind of information we can extract
>> from images that we cannot from amplitudes, intensities (even
>> unmerged)? Does anybody have a convincing argument for favour of
>> images?
>
> Overlooked superlattice?
> Incorrect point group assignment?
> Failure to recognize a non-merohedral twin?
> Thermal diffuse scatter?
> Subsequent improvements in integration programs?
>
> 	Ethan
>
>
>>
>> regards
>> Garib
>>
>>
>>
>> On 18 Mar 2009, at 16:32, Herbert J. Bernstein wrote:
>>
>>> Actually the radiologists who manage CT and PET scans of brains do
>>> have
>>> a solution, called DICOM, see http://medical.nema.org/.  If we work
>>> together as a community we should be able to do as well as the
>>> rocket scientists and the brain surgeons' radiologists, perhaps even
>>> better. -- Herbert
>>>
>>> =====================================================
>>> Herbert J. Bernstein, Professor of Computer Science
>>>  Dowling College, Kramer Science Center, KSC 121
>>>       Idle Hour Blvd, Oakdale, NY, 11769
>>>
>>>                +1-631-244-3035
>>>                [log in to unmask]
>>> =====================================================
>>>
>>> On Wed, 18 Mar 2009, Jacob Keller wrote:
>>>
>>>> Apparently it DOES take a rocket scientist to solve this problem.
>>>> Maybe the brain surgeons also have a solution?
>>>>
>>>> JPK
>>>>
>>>> *******************************************
>>>> Jacob Pearson Keller
>>>> Northwestern University
>>>> Medical Scientist Training Program
>>>> Dallos Laboratory
>>>> F. Searle 1-240
>>>> 2240 Campus Drive
>>>> Evanston IL 60208
>>>> lab: 847.491.2438
>>>> cel: 773.608.9185
>>>> email: [log in to unmask]
>>>> *******************************************
>>>>
>>>> ----- Original Message ----- From: "Klaas Decanniere" <[log in to unmask]
>>>>>
>>>> To: <[log in to unmask]>
>>>> Sent: Wednesday, March 18, 2009 5:36 AM
>>>> Subject: Re: [ccp4bb] images
>>>>
>>>>
>>>>> Herbert J. Bernstein wrote:
>>>>> Other sciences have struggled with this and seem to have found an
>>>>> answer.
>>>>> Have e.g. a look at http://heasarc.nasa.gov/docs/heasarc/fits.html
>>>>> kind regards,
>>>>> Klaas
>>>>>>
>>>>>> This is a good time to start a major crystallogrpahic image
>>>>>> archiving effort.  Money may well be available now that will
>>>>>> not be
>>>>>> avialable six month from now, and we have good, if not perfect,
>>>>>> solutions available for many, if not all, of the technical issues
>>>>>> involved.  Is it really wise to let this opportunity pass us by?
>>>>>>>> The deposition of images would be possible providing some
>>>>>>>> consistent
>>>>>>>> imagecif format was agreed.
>>>>>>>> This would of course be of great use to developers for certain
>>>>>>>> pathological cases, but not I suspect much value to the user
>>>>>>>> community - I down load structure factors all the time for test
>>>>>>>> purposes but I probably would not bother to go through the data
>>>>>>>> processing, and unless there were extensive notes associated
>>>>>>>> with
>>>>>>>> each set of images I suspect it would be hard to reproduce
>>>>>>>> sensible
>>>>>>>> results.
>>>>
>>>
>>
>
>
>
> --
> Ethan A Merritt
> Biomolecular Structure Center
> University of Washington, Seattle 98195-7742
>

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