[log in to unmask] wrote:
> Hello,
>
> 1. As long as all proteins have seventy amino-acids or less and express in
> E. coli in mM concentrations - we're in business.
>
> 2. As for the question below - my favorite answer is 'It will take a week
> and ten million dollars in unmarked bills. We begin as soon as the money
> arrives.'.
>
> Artem
>
>
>> As another example, more than once I have been asked by someone if
>> they give me the sequence or name of a protein (even membrane
>> proteins), how many days would it take us to provide them the crystal
>> structure.
>>
>> Mark
>>
>> Mark J. van Raaij
>> Dpto de Bioquímica, Facultad de Farmacia
>> Universidad de Santiago
>> 15782 Santiago de Compostela
>> Spain
>> http://web.usc.es/~vanraaij/
>>
>>
>>
>>
>>
>>
>>
>> On 12 Mar 2009, at 21:57, Felix Frolow wrote:
>>
>>
>>> Dear Mark
>>> Stay calm
>>> Buzz-words come and very frequently do not stay, they go away with
>>> the artifacts they advocate...
>>>
>>> Dr Felix Frolow
>>> Professor of Structural Biology and Biotechnology
>>> Department of Molecular Microbiology
>>> and Biotechnology
>>> Tel Aviv University 69978, Israel
>>>
>>> Acta Crystallographica D, co-editor
>>>
>>> e-mail: [log in to unmask]
>>> Tel: ++972 3640 8723
>>> Fax: ++972 3640 9407
>>> Cellular: ++972 547 459 608
>>>
>>> On Mar 12, 2009, at 6:14 PM, Mark J. van Raaij wrote:
>>>
>>>
>>>> Dear All,
>>>>
>>>> a News & Views article in Nature 458, pages 37-38 of 5 March 2009
>>>> (link below) states:
>>>> "The development of structural biology WAS historically based on
>>>> the principle of divide and conquer — individual proteins were
>>>> purified to homogeneity and their atomic structures were solved in
>>>> vitro by using either X-ray crystallography or nuclear magnetic
>>>> resonance (NMR) spectroscopy. This approach WAS tremendously
>>>> successful, and led to the creation of a protein-structure databank
>>>> that currently contains more than 50,000 structures."
>>>>
>>>> I find the past tense here too much...
>>>>
>>>> Greetings,
>>>>
>>>> Mark
>>>>
>>>> Mark J. van Raaij
>>>> Dpto de Bioquímica, Facultad de Farmacia
>>>> Universidad de Santiago
>>>> 15782 Santiago de Compostela
>>>> Spain
>>>> http://web.usc.es/~vanraaij/
>>>>
>>>> http://www.nature.com/nature/journal/v458/n723
>>>> 4/full/458037a.html
>>>> Structural biology: Inside the living cell
>>>>
>
>
And the work will be carried out in the Caribbean...
Subbu
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