I have purified my protein granulocyte colony stimulating factor using
chromatography steps. my protein is relatively pure when analysed by
reducing and non-reducing SDS PAGE method. however non-reducing page
shows DIMER presence. i have about 250 ml pure protein sample and do not
want to perform gel permeation chromatography as it will be time consuming.
is there a way to get rid of dimers by some other method. can dialysis help
me. kindly guide me as i have to formulate the product and fill it quickly.
Thanks in anticipation.
meg
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