Dear all,
sorry for the non-CCP4 question. I am expressing a protein in insect
cells that is targeted for secretion into the medium. After expression,
I want to concentrate the medium containing my protein of interest (from
10 l to 0.3 L) and at the same time change the buffer to something that
is compatible with my first ion exchange chromatography step. I would be
interested to know what kind of ultrafiltration devices can be used for
this purpose, and whether some of you could suggest a quick, reliable
and efficient system that we could purchase for our lab.
Thanks!
Michael
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