the tandem KH domain of FMRP crystallized as a very convincing dimer
(valverde et al 2007), but is a monomer in solution, although it is
not the whole protein but just two domains of it.. anyway, i would
think these ar much more common than the other way around.
Tommi
Quoting "Poul Nissen" <[log in to unmask]>:
> I would say that all crystals represent hyper-oligomeric structures,
> but never mind, I know what you mean ;-)
>
> the E. coli EF-Tu:EF-Ts complex is a good example - the structure
> clearly indicates an (EF-Tu:EF-Ts)2 dimer, and the T. thermophilus
> EF-Tu:EF-Ts is even a disulphide-linked dimer.
> However, all solution studies indicate that the E.coli EF-Tu:EF-Ts
> complex is in fact a monomeric complex.
>
> Poul
> On 11/12/2008, at 17.09, Santarsiero, Bernard D. wrote:
>
>> In parallel with the discussion around this off-CCP4-topic, are they any
>> good examples of the opposite case, where the protein is a monomer in
>> solution (as evident from light scattering, MW determination through
>> centrifugation, EPR, etc.) but crystallizes as a dimer or higher multimer?
>>
>> Bernie Santarsiero
>>
>
>
--
Tommi Kajander, Ph.D.
Macromolecular X-ray Crystallography
Research Program in Structural Biology and Biophysics
Institute of Biotechnology
P.O. Box 65 (Street address: Viikinkaari 1, 4th floor)
University of Helsinki
FIN-00014 Helsinki, Finland
Tel. +358-9-191 58903
Fax +358-9-191 59940
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