Dear Jacob,
You can find several protocols for Se-Met labeling at:
http://www.doe-mbi.ucla.edu/local/protocols
Good Luck,
Art
> Dear Crystallographers,
>
> I have been using an E coli strain called Tuner (DE3) pLacI, which is not
> auxotrophic for methionine, nor is there an auxotrophic analogue
> available.
> It may be that my protein can be expressed in other strains, but this one
> works well right now, and it is usually better to stay with what works
> well.
> Has anybody tried growing semet protein in non-auxotrophic strains, or are
> there tricks to using non-auxotrophs for semet protein?
>
> Thanks,
>
> Jacob
>
> *******************************************
> Jacob Pearson Keller
> Northwestern University
> Medical Scientist Training Program
> Dallos Laboratory
> F. Searle 1-240
> 2240 Campus Drive
> Evanston IL 60208
> lab: 847.491.2438
> cel: 773.608.9185
> email: [log in to unmask]
> *******************************************
>
Arthur Laganowsky, M.S.
David Eisenberg Laboratory
University of California, Los Angeles
Chemistry and Biochemistry Graduate Student
611 Charles E. Young Drive East
219 Boyer
Los Angeles, California 90095
USA
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