A protein band around 25 kDa could be also the
chloramphenicolacetyltransferase which is expressed in E.coli RIL/RP cells
for the coselection of the rare codon plasmids.
MS woud give the soution and it may help to identify the potential cleavage
site between GST and fused protein.
--
Christian Roth
Institut für Bioanalytische Chemie
Biotechnologisch-Biomedizinisches Zentrum
Fakultät für Chemie und Mineralogie
Universität Leipzig
Deutscher Platz 5
04103 Leipzig
Telefon: +49 (0)341 97 31316
Fax: +49 (0)341 97 31319
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