Exactly.
There are about fifteen reasons that I could name without thinking as to why
any particular step in restriction-based cloning may not work. If I think
about it the number goes up to thirty or more. Yet only ONE of them is what
actually plagues you. So please Vijay, give us sufficient details to suggest
something reasonable.
Artem
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Frank
von Delft
Sent: Saturday, August 30, 2008 7:24 AM
To: [log in to unmask]
Subject: Re: [ccp4bb] hi
PS. Sorry, couldn't resist. But seriously, for those new to the list:
if you want someone to spend time writing a useful answer, then you need
to spend time formulating a useful question.
Good: specific questions with sufficient background given
Bad: general questions which are best answered by (a) a textbook/manual
or (b) doing a PhD on it.
phx.
vijay srivastava wrote:
> hi
> i am facing problem in cloning,getting my insert and vector at the
> correct position after digestion but after ligation colony is not
> coming and if how it is coming than i am not getting my insert
>
>
>
> ------------------------------------------------------------------------
> Unlimited freedom, unlimited storage. Get it now
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