So what, then, will be the concentration of the acetate ion in your
stock solution when you have finished?
(Disclaimer: I get to teach this stuff periodically in remedial
chemistry as a punishment for deployment of excessive sarcasm during
faculty meetings.)
On Jul 22, 2008, at 6:10 AM, Santosh wrote:
> Hi,
> Make a 1M Na-Acetate do not make up to the 1 Ltr volume. Leave some
> extra
> volume and now start adding Acetic acid till you get pH 4.5 (Glacial
> Acetic
> Acid).
> Now make up the volume to 1ltr or how much ever you are deciding to
> make the
> 50X stock solution.
> Best,
> Santosh
>
> On Mon, Jul 21, 2008 at 11:20 PM, William G. Scott <
> [log in to unmask]> wrote:
>
>> This is a job for the trusty Henderson-Hasselbalch equation:
>>
>> http://en.wikipedia.org/wiki/Henderson-Hasselbalch_equation
>>
>>
>>
>> On Jul 21, 2008, at 8:12 PM, Meg wrote:
>>
>> Dear All,
>>>
>>> I want to prepare 25 mM sodium acetate buffer pH 4.5. can anyone
>>> give the
>>> exact composition of how to prepare it. we prepare it using sodium
>>> acetate
>>> and acetic acid combination. i am not able to arrive at the
>>> calculatation
>>> correctly, so if anyone can explain me with the above buffer how to
>>> calculate. and what sodium acetate [Anhydrous / trihydrate] and
>>> acetic
>>> acid
>>> [glacial/ plain] to use.
>>>
>>> thanks n regards
>>>
>>> Meg goyal,
>>> M.SC Biotechnology [Research]
>>> Institute of science,
>>> Fort
>>> Mumbai, INDIA
>>>
>>
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