A mild and quick method is to use dry Sephadex G-25. The material will
swell and take up all the liquid except molecules larger than ca. 5 kDa.
> Dear All,
>
> we have GCSF protein produced in inclusion bodies. we solubilise it refold
> it and then concentrate it using proflux system. still the concentration
> of the protein we get is less and volume is more for us to load in Ion
> exchange chromatography. is there any simple technique that can be
> performed in lab without using any hi-fi instrument to concentrate the
> protein in small volume of buffer. the protein we obtain is about 0.7
> mg/ml and we get 450 ml solution. our column is 110ml lab scale and we
> have to work in that only. i have heard of NH4SO4 precipitation. but it
> requires protein conc more than 1 mg/ml.
>
> kindly help me to progress in my experiment.
>
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