Hi Mike,
how does your protein look on a sizing column ? Perhaps a dimer ? That
would then explain the tendency to form dimers in different crystal
forms, as it is a biological relevant dimer. for the eventuality that
you have no clue how the sizing looks like, run one and find out.
Juergen
Michael Colaneri wrote:
> Dear colleagues,
>
> I have a structure with two identical dimers per asymmetric unit. If
> the dimers are identical in different crystalline environments and in
> different crystal forms they should be particularly stable.
>
> How does such a stable dimer crystallize? Is it necessay to pre-exist
> in the specific conformation in solution (even to a lesser extent than
> a major monomeric form) or a very stable dimer can assemble from the
> very beginning durnig crystallization? And can the same dimer form
> during crystallization in different crystalline environments without
> it being present in solution to any (even infinitesimal) extent?
>
> I would appreciate all responses.
>
> Thanks.
>
> Mike Colaneri
--
Jürgen Bosch
University of Washington
Dept. of Biochemistry, K-426
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4510
FAX: +1-206-685-7002
Web: http://faculty.washington.edu/jbosch
|