>>With regard to low levels of expression: Is there a particular reason
>>to use 293 cells? Transient expression in Cos-1/Cos-7 cells gives
>>~ 10 fold higher levels of expression because of the plasmid amplification
>>in these cells. (The only requirement is that the plasmid should
>>have SV40 ori; most mammalian vectors do).
>
>HEK293T cells are reported to have high expression levels, especially
>for secretted proteins (Aricescu et al., ACTA D, 2006).
Ah, yes, indeed. Like Cos cells, they do express large SV40 antigen,
so the transient expression levels will be high. I did not know about
it, thinking of "normal" 293 fibroblast line.
Dima
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