from the horses mouth ..
----- Forwarded message from [log in to unmask] -----
Date: Mon, 03 Mar 2008 10:16:41 +0000
From: Alexander Golovanov <[log in to unmask]>
Reply-To: Alexander Golovanov <[log in to unmask]>
Subject: Re: Fwd: Re: [ccp4bb] Glu+Arg solutions for solubility
To: Stephen Prince <[log in to unmask]>
Don't use salts, as it says (otherwise you will have to titrate pH a
lot to get it back to neutral). Just L-Arg and L-Glu. First, put
L-Arginine powder in some water, start dissolving it, then add L-Glu
powder, stir them together, top up the volume as required. They are
only soluble together at 1 M. The final pH of the 1M stock will be
(by itself) around 6.4 or close to neutral, in our experience.
good luck!
Sasha
At 09:17 03/03/2008, you wrote:
> ... from a bulletin board I subscribe to, are there any tricks for
> making the stock (pH?) ...
>
>
> On Thu, February 28, 2008 4:33 pm, Paul Paukstelis wrote:
>> posting for a colleague:
>>
>> In Golovanov et al. 2004 they indicate it is possible to make a 1M
>> solution of free amino acids (not amino acid salts) Glu+Arg in the
>> context of generating solutions for keeping proteins soluble. Has anyone
>> been able to do this?
>>
>
>
> ----- End forwarded message -----
>
>
> ______________________________________________________________________
>
> S.M. Prince Phone: +44 (0)161 306 8919
> Faculty of Life Sciences FAX: +44 (0)161 306 5201
> Manchester Interdisciplinary Biocentre
> University of Manchester
> 131 Princess Street e-mail: [log in to unmask]
> Manchester
> M1 7DN
> U.K.
> ______________________________________________________________________
--
Dr Alexander 'Sasha' Golovanov
Lecturer
Faculty of Life Sciences and
Manchester Interdisciplinary Biocentre
The University of Manchester
131 Princess Street
Manchester M1 7DN, UK
Tel. No: +44 (0) 161 306 5813 Office (G.024)
+44 (0) 161 306 5812 NMR room
FAX: +44 (0) 161 306 5201
e-mail [log in to unmask]
web: http://www.mib.manchester.ac.uk/aboutus/people/details.aspx?val=97941
----- End forwarded message -----
______________________________________________________________________
S.M. Prince Phone: +44 (0)161 306 8919
Faculty of Life Sciences FAX: +44 (0)161 306 5201
Manchester Interdisciplinary Biocentre
University of Manchester
131 Princess Street e-mail: [log in to unmask]
Manchester
M1 7DN
U.K.
______________________________________________________________________
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