I have not tried to make 1M solutions of the indicated AA, but i have had
good luck of using what they suggest in that specific paper. By using 50mM
of each (Glu and Arg) I was able to increase the solubility of a 44kDa
protein (a Serpin) from ~1mg/ml to above 17mg/ml. So in my case it really
worked. This was for the purpose of doing liquid state NMR on that
protein.
I does help to solubilize the two aa in 1:1 ratio as the two combined
improve their individual solubility.
Jan Jensen
On Thu, February 28, 2008 4:33 pm, Paul Paukstelis wrote:
> posting for a colleague:
>
> In Golovanov et al. 2004 they indicate it is possible to make a 1M
> solution of free amino acids (not amino acid salts) Glu+Arg in the
> context of generating solutions for keeping proteins soluble. Has anyone
> been able to do this?
>
>
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