Most reagents that modify side chains are also (not surprisingly) toxic.
Many of them are small and therefore membrane-permeable.
Look up recent works on in vivo 'click chemistry'. Unfortunately I doubt
that these compounds can be classified as 'cheap'.
If you want to do this in vivo, you're probably better off using biology
rather than chemistry - i.e. enzyme tagging. If you are able to disclose
more details on what are you trying to accomplish perhaps this forum may be
of better use :)
Artem
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Jacob
Keller
Sent: Thursday, February 21, 2008 5:32 PM
To: [log in to unmask]
Subject: [ccp4bb] Side chain chemical modifiers
Dear Crystallographers,
I am looking for a way to modify any specific set of sidechains in my
protein (e.g., all K's, or all D's and E's, etc.). A few requirements:
1. Relatively cheap
2. Definitely non-cell-membrane-crossing
3. Specific
4. Any reasonable amount of change in mass (+ 5-5000 Da?), charge, etc., is
fine
5. Preferably less life threatening to live cells (I know this is a probably
a losing battle)
The last criterion is because I want to modify the protein in vivo, then
immediately harvest the cells, etc. I was hoping there would be something
"around the house" that I could use for this, especially for hitting K's.
All the best,
Jacob
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: [log in to unmask]
*******************************************
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