Dear CCP4s
I am trying to solve a structure for which I have two datasets: One comes from a selenomethionine derivatized crystal at 3 A resolution and the second comes from a native one that gave 2.6A. I have obtained workable experimental phases by SAD using SOLVE/RESOLVE. With this I have built a model and obtained a calculated map using REFMAC and the native dataset. I cannot push the Rfree further than 34.5 (Rw:28) and given the low quality of the original density I ended up having 6.5% outliers. I was told there is an option with which I could start from scratch calculating a new map using "phase combination" between the derivatized dataset and the model.
Could anyobody clarify further what phase combination is and which are the programs for that purpose?
Regards
Rafael (thats my real name)
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