> >The problem may be that the reference experiment type in CCPN has the
> >dimensions in the wrong order, and so some reimported ARIA dims are getting
> >swapped. However, this can be set Experiment::Experiment Types:|Ref Exp
> >Dim| - There is no a priori way of the software necessarily knowing which
> >dim is which. But if you look though the Ref Measurements you can see
> >which dim is which with regards to the magnetisation transfer, so for
> >example with an H[N]_H.NOESY the transfer goes 1) Hn - 2) N - 3) Hn - 4)
> >Hx so the Hn is the dim measured at steps 1) & 3) and Hx at step 4).
> >If you swap these reference dims or set the experiment type you can use
> >the resonance quality report to see if the bound resonances make sense.
>
> Yes, I was looking into this as well. Though I was puzzled how my NOESY is
> treated. I have got a gnoesyNhsqc experiment (in Varian terminology), that
> is an HSQC step goes after noe transfer. Thus I have selected an
> H_H[N].NOESY. My understanding is that the transfer should be set to
> Hx->Hn->N->Hn, but there is no such an option in the Edit Experiment Types
> table, is there?
What you have selected does seem like the correct experiment type, for the
correct transfer pathway, but any problem is probably to do with the order
of dimensions; i.e. which 1H dim in the spectrum binary data goes with
which of the dimensions of the pathway. Even if the pathway is definitely
Hx-Hn-N-Hn in the pulse experiment then this says nothing definte about
the way that the data is eventually stored.
Usually the fix is as simple as swapping the 1H Ref Exp Dims.
T.
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Dr Tim Stevens Email: [log in to unmask]
Department of Biochemistry [log in to unmask]
University of Cambridge Phone: +44 1223 766018 (office)
80 Tennis Court Road +44 7816 338275 (mobile)
Old Addenbrooke's Site +44 1223 364613 (home)
Cambridge CB2 1GA WWWeb: http://www.bio.cam.ac.uk/~tjs23
United Kingdom http://www.pantonia.co.uk
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