Have you tried TLS and NCS? Maybe these can lower the R free a little.
But ,the quality of your model depends on the quality of your data and
completeness of your model etc.
Yongchao Li wrote:
>> I have a large molecule to refine.There are about three thousand
>> residues in one asymmetric unit. Except for the lack of electron
>> densities in several places, where the loops have been deleted, the
>> rest parts fits well with density. But the R free and R work are all
>> beyond 0.30 (R free = 0.35, R work = 0.30).
>> I don't know how to reduce them. Can anyone give me some ideas?
>>
>> Thank you!
>>
>>
>> ---------------------------------
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>
>
> Size alone is not a problem.. think of viruses
> But it is easy to make errors of course, and more time consuming to
> check in such a long chain
> what is the resolution though..
>
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