You could, for example, try and increase the precipitant concentration
after the crystals have grown. This might stabilise them even at room
temperature and may also improve their quality.
If you cover your trays with tape, you can easily exchange the reservoir
solution with a syringe without having to transfer the tray.
Tim
--
Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
On Tue, 11 Dec 2007, wrote:
>
> Now I have a problem,one enzyme crstallized using hanging-drop method at 32 degree,and it is very sensitive. If I want to check the plates at room temperature,it will disappear very soon,when I put back the plates to 32 degree,crystals will come again,so we only can freeze the crystals for measurment.Unfortunatly ,the resolution always around 5 or 6 A.Is there anybody can give some suggestion?Thank you very much!Liuqing Yang
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