Dear all,
Sorry for bothering you. I have an off-topic question about how to choose a suitable expression vector and strain to overexpress mitochondria protein in E Coli for the future crystallographic study.
It is a human metabolic enzyme expressed in the liver. My colleagues tried to overexpress it in E. Coli(XL-blue), however there was no activity observed. I am wondering if anybody know there are vectors and starins which are good for mitochondria protein expression. Or you can give me general suggestions on choosing protein expression system in E. Coli. I am a graduate student starting to learn molecular biology. I greatly appreciate your help in advance.
Best wishes
Wei Yong
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