A few week ago I posted a question asking about the recommended time from collection to separation of samples for P3NP. Below is the mailbase reply.
Thanks,
Anna
Dear Anna,
Your query was passed on to me as we run a PIIINP assay service at
Manchester Royal Infirmary.
We routinely recommend that samples should be separated within 2 hours
of being taken, as is usual for routine samples in most labs. Orion, the
manufacturers of the PIIINP assay, do not make a recommendation in their
assay insert but when contacted, said that they had never looked at the
effect of prolonged time to separation (beyond 2 hours). Personally, on
the basis of experience and published work with other analytes I would
not be too concerned if the separation time went up to 3-4 hours but we
will have to do the work to get some hard evidence.
As far as storage after separation is concerned we looked at this many
years ago and there is little problem. Samples will show no significant
change over at least 12 months if stored at -20C and in the fridge will
show no significant change over 7 days. Even at room temperature, we
found only a small decrease (<10%) in PIIINP levels after 7 days.
Let me know if I can be of any more help.
Regards,
Sandy Smith
Dr Alexander Smith B.Sc., Ph.D.
Principal Clinical Scientist
Clinical Research Department, Manchester Royal Infirmary
Miss Anna Barton
Locum Senior Biochemist
Royal Cornwall Hospital, Truro
[log in to unmask]
Tel: 01872 252837
>>> Anna Barton <[log in to unmask]> 27/09/2007 12:14:15 >>>
We are currently sending away samples for P3NP. So far patients have been attending hospital clinics, so time from collection to separation & freezing has been short.
Now our clinicians would like some samples to be taken at local outpatient clinics and sent on via the courier to our laboratory, obviously increasing time from collection to separation &/or storage
We would like to know what advice to give on these samples:
- Is there a recommended time from sample collection to centrifugation/separation?
- Plus, is there is a recommended time from separation to storage?
eg. could a clinic take & separate a sample ready to await courier collection & delivery to the lab.
I have looked on assayfinder however many of the entries only cover storage requirements rather than information on separation and time to storage etc.
Any information would be great and I'm happy to collate the information for re-posting on to the mailbase.
Thanks,
Anna
Miss Anna Barton
Locum Senior Biochemist
Royal Cornwall Hospital, Truro
[log in to unmask]
Tel: 01872 252837
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