I don't have the quantitative data any more, but interferences in the
Biuret method depend on the concentration of protein at which you are
measuring and, at the concntrations found in serum, glucose is not a
significant interfering substance. Sucrose can cause interference if
present at high enough concentration (this would be from an ex-vivo
sucrose gradient, rather than a physiological situation). Lipids
interfere by causing turbidity and hence the recomended "long"
procedure of decolourising with KCN after the reaction and re-reading
the blank (I doubt that many people do that any more). Reference
Gornall AG et al J Bio,Chem 1949 177:751-766 if you want to read the
On 13/09/2007, Dr. Michael Steiner <[log in to unmask]> wrote:
> Dear Lab Community,
> Is the interference of hyperglycaemia on the Biuret protein assay a real
> thing or a "may be" textbook statement ? Any quantitative data (most
> probably from the preceding century ...)?
> This question came up while discussing total protein methodology.
> Thank you for your input.
> Michael Steiner
> Institute of Clinical Chemistry & Laboratory Medicine
> University of Rostock
> D-18057 Rostock
> ------ACB discussion List Information--------
> This is an open discussion list for the academic and clinical
> community working in clinical biochemistry.
> Please note, archived messages are public and can be viewed
> via the internet. Views expressed are those of the individual and
> they are responsible for all message content.
> ACB Web Site
> List Archives
> List Instructions (How to leave etc.)
------ACB discussion List Information--------
This is an open discussion list for the academic and clinical
community working in clinical biochemistry.
Please note, archived messages are public and can be viewed
via the internet. Views expressed are those of the individual and
they are responsible for all message content.
ACB Web Site
List Instructions (How to leave etc.)