Hi all,
Apologies if this has been asked before. I am currently refining a
structure with 4 molecules per ASU. While all 4 molecules are the
same protein, they do not share 4-fold NCS due to a slight hinge
movement - the ASU actually contains two times 2-fold NCS. As this is
a big structure at low res I would really like to use the automagic
NCS-averaged maps generated by coot. However, when I try to do this
coot thinks that all 4 molecules are identical and does 4-fold
averaging accordingly.
How does one 'tell' coot what the NCS relationships are between chains?
Thanks,
Stephen
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Dr Stephen Graham
Nuffield Medical Fellow
Division of Structural Biology
Wellcome Trust Centre for Human Genetics
Roosevelt Drive
Oxford OX3 7BN
United Kingdom
Phone: +44 1865 287 549
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