A possible modification for cysteine that adds extra density is S-(dimethylarsenic) cysteine (CAS). Requires DTT and cacodylate buffer conditions however. And does not crosslink so far as I know.
Has been seen in a number of structures from cacodylate conditions - eg. one of the Xrcc4 structures
cheers
Martyn
Martyn Symmons
Department of Pathology
University of Cambridge
========================================
Message Received: Apr 12 2007, 06:06 PM
From: "Flip Hoedemaeker" <[log in to unmask]>
To: [log in to unmask]
Cc:
Subject: Re: [ccp4bb] oxidised cys
I've actually seen something like this on disulfides (or at least I think
so, I havent seen your density obviously), turned out it was model bias in
MR, if I used a different template for MR the feature went away. This was
high resolution stuff (~1.0 Å).
Flip
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
[log in to unmask]
Sent: Tuesday, April 10, 2007 20:44
To: [log in to unmask]
Subject: Re: [ccp4bb] oxidised cys
Hi Stefano,
How certain are you that this link is truly what you think it is? If I
understand what you're saying - you want to create a (thioperoxythio) link
- this chemistry should be hideously unstable. Can you explain this using
disorder, or perhaps the residual density is a symmetry artifact?
Regards,
Artem
> Dear all
> in my structure I think I can see an oxidised Cys in cys-SO. Refining
> cys-SO
> I observe a residual density between the oxigen of one oxidised cys and
> the
> one of the other molecule in AU.
> I'd like to try to refine it as cys-SO-OS-cys. I didn't find an example of
> it in the pdb database. Could anyone tell me whether there are other
> cases?
> I guess I just didn't find them.
> Second question:
> How could I "explain" to refmac that there is the OO bond?
> I tried to write a line similar to the one for SSBOND in the pdb header
> OOBOND 1 CEA A 42 CEA D 42
> but refmac couldn't care less...
>
> thanks in advance
>
> Stefano
>
> _________________________________________________________________
> Express yourself instantly with MSN Messenger! Download today it's FREE!
> http://messenger.msn.click-url.com/go/onm00200471ave/direct/01/
>
|