Dear friends,
Recently, I have solved a structure using mad method. When using the peak
data(2.3A) as the native for structure refinement, the gap between R
factor and R free is big, about 0.1(0.22 and 0.32). I modelled the
selenomethionine but the gap still exists. When I changed the data for a
real native one(2.7A),it seems OK with R=0.24 and Rf=0.28.
Does anyone have the similar experience?
what should I pay attention to when using the sad/mad data as the native
one for modelling and refinement?
Thanks in advance.
--
Peng Zhang, Ph.D. Student
Institute of Biochemistry and Cell Biology
Shanghai Institutes for Biological Sciences
Chinese Academy of Sciences
320 Yue-Yang Road
Shanghai 20031
P.R. China
Tel: 021-5492-1117
Fax: 021-5492-1116
Email: [log in to unmask]
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