> for ground state ATP analogues use AMP-PNP (or AMP-PCP) or ADP in complex
> with beryllium fluoride.
In some circumstances ATP-gamma-S will work too. It's less resistant to
hydrolysis, so it's a bad idea for many enzymes, but the resulting active
site geometry can be different depending on which analogue is used. I
know of at least one case where independent structures of the same protein
had different nucleotide conformations due to the choices of analogue
(ATPgS vs. AMPPNP).
-Nat
|