Hi Zhiyi,
for ground state ATP analogues use AMP-PNP (or AMP-PCP) or ADP in complex
with beryllium fluoride.
transition state like analogues can be formed with ADP in complex with
aluminium fluoride or magnesium fluoride. ADP - nitrate has also been
used as a transition state analogue, hope this helps, cheers, Matt
Matthew Bowler
MRC Dunn Human Nutrition Unit
Wellcome Trust / MRC Building
Hills Rd
Cambridge
CB2 2XY
> Hi everyone,
>
> I want to know which ATP analogues are suitable and popular for
> co-crystallization
> with enzyme.
> Thank you in advance.
>
> Best,
> Zhiyi
>
>
>
>
> ------------------------------
> Zhiyi Wei
>
> ---------------------------------------------
>
> Institute of Biophysics, Chinese Academy of Sciences
>
> 15 Datun Road, Chaoyang District
>
> Beijing, P.R.China, 100101
>
> Tel:86-(0)10-64888465
>
> Fax:86-(0)10-64888513
>
> ---------------------------------------------
>
> School of Life Sciences,
>
> University of Science & Technology of China,
>
> Hefei, Anhui, P.R.China, 230026
>
>
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