Hello,
The fitting routine estimates the rate using whatever function you have
chosen to do the fitting. TC is then 1/rate. That is pretty
non-controversial. The TC error is trickier. The same code that
estimates the rate also estimates the error to the rate using a
"bootstrap" method (as of version 1.0.9). Specifically, if you have n
data points (each x, y), then the program repeatedly (niter times) chooses
n points out of the n but with repeats allowed. Each of those gives a
fit, so we end up with a distribution of rates, and the rate error is
estimated to be the standard deviation of that distribution divided by the
sqrt(niter-1). Then from that estimate of the rate error you get an
estimate of the TC error by:
TCerror = 0.5 * (1.0/(rate-rateError) - 1.0/(rate+rateError))
All in all this is slightly dubious. I would trust the TC but not so much
the TC error. I guess a Bayesian analysis might be better.
Wayne
On Fri, 24 Feb 2006, Karthik Venkatesh Rajasekar wrote:
> Hello!
>
> I have tried to determine the T1 nd T2 using the Rates analysis function in
> CCPN. Can someone tell me what TC and TC error is.
>
> karthik
>
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