Dear Tim,
In the HSQC spectra, window2 (F1 1H, F2 15N, F3 1H), I selected a
peak and assigned it anonymously as {137}H[304] {137}N[305]. Then, I
picked a corresponding CB and CA peak from a CBCA(CO)NH spectra in
window3 (F1 1H, F2 13C, F3 15N). I set the sequential spin system to
F1 0, F2 -1, F3 0 and propagated the NH assignment. The I selected a
peak, say, CB and assigned an atom type as CB and added to spin
system. it got the same spin system, {137}H[304] {137}N[305] {137}CB
[306]! I have tried this in few different possible orders, like
propagating the NH assignment and then setting sequential spin
system. It does not seem to help!
I need this functionality to continue using it!!
Many thanks.
Best,
Murali.
I am using v 1.0.7. The only difference between your version and mine
should be in gl_handler.c where I have gldeletelist commented out.
Anyway, i am using only Tk as the handler and I can't imagine why
that should affect this?!
On 12 Dec 2005, at 07:18 pm, Tim Stevens wrote:
>> I have three windows as follows:
>> window1 - triple resonance experiments + 15N-HSQC - x/F1:1H, y/
>> F2:15N, z/F3:13C
>> window3 - triple resonance experiments - x/F1:1H, y/F2:13C, z/F3:15N
>>
>> I have followed exactly, as it is said (or at least as I have
>> understood!) in the advanced tutorial, and tried to set the
>> sequential spin system for CBCA(CO)NH peaks as F1: 0, F2: -1, F3: 0
>> (in window3). When click add to spin system, I do not see two
>> different spin systems for the amide and carbon plane. Both gets the
>> same spin system. What am I doing wrong here? I want to create a list
>> of anonymous connected peaks like this. It does not look easy as it
>> should be?
>
> The "Add to spin system" function, as you've observed, sets all
> resonances
> for a peak to be in the same spin system, and makes a new spin
> system if
> none is present for the peak. Useful for HSQC etc., when you're
> working
> within one residue.
>
> The "Set sequential spin systems" is different in that it will
> create two
> connected spin systems, according to the label "F1: 0, F2: -1, F3:
> 0" etc.
>
> I just tried this out on an H[N[co[CA]]] experiment and the initial
> assignment changed from:
>
> F1 F2 F3
>
> {69}[137] [238] {69}[138]
>
> to:
>
> {69}[137] {95}[238] {69}[138]
>
>
> So it seems to have successfully made a new i-1 spin system for my
> carbon
> dimension.
>
> Maybe this didn't work as it should when you tried. I seem to
> remember a
> bug fix in this area a little while ago...
>
> To help me pin down the problem, what is the assignment label of an
> example peak?
>
> T.
>
> ----------------------------------------------------------------------
> ---------
> Dr Tim Stevens Email: [log in to unmask]
> Department of Biochemistry [log in to unmask]
> University of Cambridge Phone: +44 1223 766022 (office)
> 80 Tennis Court Road +44 7816 338275 (mobile)
> Old Addenbrooke's Site +44 1223 364613 (home)
> Cambridge CB2 1GA WWWeb: http://www.bio.cam.ac.uk/~tjs23
> United Kingdom http://www.pantonia.co.uk
> ----------------------------------------------------------------------
> ---------
> ------ +NH3CH(CH(CH3)OH)C(O)NHCH(CH(CH3)CH2CH3)C(O)NHCH(CH2CH2SCH3)
> CO2- -------
> ----------------------------------------------------------------------
> ---------
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