Dear allstat
I hope someone will be able to help me.
I have been asked to reproduce a 4 parameter curve fit for analyte
signal vs concentration. The curve has already been defined by the
machine that produced the signals and so I have the parameter values
that define the curve but do not know the function that relates signal
to concentration.
The output from the machine reports that a "semi-log sigmoid fit with
tails" was used. I have looked through the internet and deduced that
semi log indicates that one of the variables is logged. This seems to
tie in with a plot I saw (of different data but same assay) where the
concentration was plotted in the logarithmic scale (X axis).
My understanding in that a sigmoid curve is just one that looks like an
'S' and is a special case of the logistic function. Is it possible that
the term sigmoid is being used as a general term and may include the
logistic function?
In SAS JMP I found a 4 parameter logistic function which was S-shaped,
although when I used it to fit the data I am getting nothing meaningful
and so am currently messing around with various parameter values to try
and get a reasonable fit. There is also a growth curve which looks as
though it may be a possible candidate, but I haven't tried it yet.
I suppose there are few questions that I have:
Does anyone know what the function would be for a "semi-log sigmoid fit
with tails" to model signal as a function of log concentration?
Is it reasonable to use an alternative 4 parameter equation even though
it is not the same as the one already evaluated?
Is there only one form of the logistic 4 parameter curve fit?
Any help would be gratefully appreciated.
Regards,
Jayne
|