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PLANT-HORMONES  2000

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Subject:

Re:IBA and abscission

From:

steve croker <[log in to unmask]>

Reply-To:

[log in to unmask]

Date:

Tue, 3 Oct 2000 10:05:04 +0100

Content-Type:

text/plain

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text/plain (97 lines)

Moderated for plant-hormones by Steve Croker Long Aston Research Station
BBSRC

> Marcos,
>
> The 3% does sound whopping, but if you think
> about it, you are applying only a tiny amount
> of the lanolin paste to the surface. 3% of that
> is a very tiny amount. Some will never leave the
> lanolin, some will never cross into the tissues,
> some will be oxidized and enzymatically degraded,
> some will be active. So, I'm guessing that 3% is
> indeed the recommended amount. I have always
> made up 0.5% for other purposes...not for hibiscus.
>
> Procedurally, you weigh out your IBA for the amount
> you want to make (3 grams IBA per 100 grams final for
> example) into a Pyrex container. Heat your anhydrous
> lanolin supply until it is a pourable liquid; a water bath
> is good for that purpose. Then you dissolve the
> IBA in the Pyrex container in a few drops of alcohol
> or acetone. Once it is completely dissolved, quickly
> weigh the container on a balance and pour in the
> required amount of liquefied lanolin (in our example
> 97 grams). You want to stir thoroughly as the lanolin
> cools and becomes a nice paste. I then keep mine
> in the refrigerator and warm the container in my hands
> to soften the lanolin just before use. A glass rod is good
> for the stirring, but I use disposable wood picks for the
> applications to plants.
>
> In hormone biology, typically orders-of-magnitude
> differences are required to see measurable differences.
> So, if you suspected that 3% was too high, you could
> take 10 grams of what you made above and mix it
> thoroughly with 90 grams of anhydrous lanolin to make
> a 0.3% paste. You could take 10 grams of that 0.3%
> and mix it with 90 grams of anhydrous lanolin to make
> a 0.03% paste. These three would probably give you
> one which worked VERY nicely I'm guessing.
>
> Best wishes,
>
> ross
>
> Ross E. Koning, PhD
> Professor of Biology - Goddard Hall
> Eastern Connecticut State University
> Willimantic, CT 06226 USA
> Pager: (860)-744-2705 (leave return number at beep)
> Office: (860)-465-5327
> Home: (860)-423-9724
> Email: [log in to unmask]
> Home: [log in to unmask]
> http://koning.ecsu.ctstateu.edu
>
>
> > ----------
> > From: steve croker
> > Reply To: [log in to unmask]
> > Sent: Monday, October 2, 2000 4:21 AM
> > To: Plant Hormones (E-mail)
> > Subject: IBA and abscission
> >
> > Moderated for plant-hormones by Steve Croker Long Aston Research Station
> > BBSRC
> >
> > I understand this list is meant to be used by scientists, but I hope
> > you won't mind if a layman gardener ask a question too:
> >
> > I intend to use IBA water soluble salts to stabilise Hibiscus
> > rosa-sinensis
> > pods, as they frequently drop at half or 2/3rd term. I have read in a
> > specialised hibiscus publication that a mix of lanolin and 3% IBA may
> > be used for this purpose, but I wasn't able to find further information
> on
> > this issue. Particularly, there was no explanation as to the proper
> ratio
> > of
> > lanolin:IBA solution , and I suspect a 3% concentration of IBA would
> > be too much for a plant hormone.
> >
> > Any information will be greatly appreciated.
> >
> > Thanks in advance,
> >
> > Marcos Capelini
> > [log in to unmask]
> > São Paulo
> > Brazil
> >
> >
>
>


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