On 17/05/12 14:48, Ed Pozharski wrote:
> On Thu, 2012-05-17 at 14:13 +0530, Faisal Tarique wrote:
>> to make disulphide bond ie to connect cysteine with bme
> While I believe that CYS+BME is a correct choice ideologically (you had
> cysteine and bme reacted with it), note that you can use CME monomer
> (it's listed as peptide in monomer libraries, so will incorporate fine).
> On the other hand, I have added BME a number of times in coot and had no
> problems. Yes, the bond is not showing, likely because coot decides
> which bonds to draw without much attention to atom type
In Coot 0.7, to draw a bond between monomers that don't have an implicit
connection due their serial number, you need a LINK record. You can add
a LINK using Extensions -> Modelling.
Coot 0.7 has an interface to (recent versions of) JLigand to make the
generation and transfer of bespoke link information considerably more
straightforward (and in so doing will generate a LINK for you).