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CCPEM  July 2018

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Subject:

Re: Knowledge question: information represented in 2D class?

From:

Sjors Scheres <[log in to unmask]>

Reply-To:

Sjors Scheres <[log in to unmask]>

Date:

Thu, 12 Jul 2018 17:44:24 +0100

Content-Type:

text/plain

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Parts/Attachments

text/plain (51 lines)

Dear Austin,
The 2D class average images should look like true projections, as they are
merely the average of many individual particles, each of which is of
course also a projection. So your interpretation of the 2D class averages
until recently was not correct: you will see 'everything' in the path of
the electrons, not just on the face of the particle.
HTH,
Sjors




> After some searching, I couldn't find an answer to this question.
>
> Up until recently I had always seen 2D classes similar to looking at
> different angles of a solid object (like I was rotating the protein in my
> hand). I see a surface. This is different from a 2D projection, which I
> have always seen as 3D density information flattened into a 2D image. In a
> projection, features have no context in the z-axis. So if you see
> something, you wouldn't be able to tell its absolute position on the
> particle, only its relative orientation.
>
> I had always seen classes as the opposite: if you see features in a 2D
> class, it is on that face of the particle.
>
> Is that true? Or do I have my understanding mixed up? Recently some of our
> classes (Relion) have shown features we expected on a different face
> (based on biochemical data).
>
> Thank you for indulging a lowly assistant :)
> - Austin Dixon
>
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>
> To unsubscribe from the CCPEM list, click the following link:
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-- 
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres

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