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Over the recent 3 weeks we have had 4 requests for lactate and pyruvate analysis. When compared with 1 request for pyruvate in the last 3 years this seamed odd ! I must confess to have only textbook knowledge of these analytes. I would welcome some comments from some of the more seasoned biochemists on the utility of the above analytes. For example what are the indications for the measurement of pyruvate. Is it unreasonable for us to insist that the requesters document an acidosis and an elevated anion gap before preceding to lactate analysis (we have been doing this in adults for sometime. Is it the same situation in neonates ?) Craig Webster Senior Clinical Biochemist Broomfield Hospital %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%