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Hi Very strange. No idea what is happening. One thing you can do is take one of those regions as a seed and set the others as targets (--targetmasks flag). The look at the output seed_to_target files. Using fslstats, you can calculate the total number of streamlines from the seed to reach each target (by multiplying the results of the -M and -V flags). These should map onto the corresponding row of the network matrix and should enable you to start debugging where the problem is occurring. Cheers Saad From: FSL - FMRIB's Software Library <[log in to unmask]> on behalf of Jürgen Hänggi <[log in to unmask]> Date: Friday, 5 August 2022 at 15:04 To: [log in to unmask] <[log in to unmask]> Subject: Re: [FSL] Maximum number of streamlines is 2^24 Dear Saad thanks for your response. I increased the number of streamlines per seed (option -P from 5000 to 6000) and again, the largest value is 2^24. With -P 6000, more connections now show this value compared with -P 5000, i.e. 98 out of 8010 connections in the matrix have a value of 2^24 and no connection showed a larger streamline count. Thanks in advance for any advice. Best regards Jürgen Am 05.08.2022 um 09:46 schrieb Saad Jbabdi <[log in to unmask]<mailto:[log in to unmask]>>: Hi That seems unlikely. If you increase the number of streamlines per seed, does that maximum also increase? Cheers Saad From: FSL - FMRIB's Software Library <[log in to unmask]<mailto:[log in to unmask]>> on behalf of Jürgen Hänggi <[log in to unmask]<mailto:[log in to unmask]>> Date: Friday, 5 August 2022 at 08:09 To: [log in to unmask]<mailto:[log in to unmask]> <[log in to unmask]<mailto:[log in to unmask]>> Subject: [FSL] Maximum number of streamlines is 2^24 Dear FSL experts we have run probtrackx2_gpu with the --network option to generate connectivity matrices among the 90 regions of the AAL atlas. we then recognized that there are a lot of identical numbers of streamlines in the matrices. This number is always the largest number in the matrix (i.e. 1.67772e+07) and it is equal (apart from the fact that there are only 5 decimals in the scientific notation of the number of streamlines in the generated connectivity matrix) to 2^24. it seems that the maximal number of streamlines is truncated to 2^24! we are wondering whether this problem is related to probtrackx2_gpu or to parameters of our terminal / Ubuntu OS. how to solve this problem? Thanks in advance for any advice. Best regards Jürgen ######################################################################## To unsubscribe from the FSL list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=FSL&A=1 This message was issued to members of www.jiscmail.ac.uk/FSL<http://www.jiscmail.ac.uk/FSL>, a mailing list hosted by www.jiscmail.ac.uk<http://www.jiscmail.ac.uk/>, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ ________________________________ To unsubscribe from the FSL list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=FSL&A=1 ________________________________ To unsubscribe from the FSL list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=FSL&A=1 ######################################################################## To unsubscribe from the FSL list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=FSL&A=1 This message was issued to members of www.jiscmail.ac.uk/FSL, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/