On Tue, Jun 28, 2022 at 5:33 PM Ioannis Skalidis <[log in to unmask]> wrote:

Marino is right, ideally, to get a map-model correlation you would use
Phenix' Cryo-EM validation suite, but I'm not sure at this resolution if
it's that meaningful either.

Cheers,

Yiannis.

On 28.06.22 10:21, Marino Jacopo (PSI) wrote:
> Hi - you can do that in Chimera, for example. But the correlation number won't mean much.
>
> Cheers
>
> -----Original Message-----
> From: Collaborative Computational Project in Electron cryo-Microscopy <[log in to unmask]> On Behalf Of Si Hoon Park
> Sent: Tuesday, 28 June 2022 10:03
> To: [log in to unmask]
> Subject: [ccpem] Atomic model fitting in cryoET map
>
> Dear CCPEM community,
> Thank you for all your efforts in the field of structural biology.
> I would like to ask you about how to fit the model into a low resolution map.
> I've got an electron density map of the protein oligomer on the lipid bilayer at ~25 A resolution.
> Although the resolution was low, the shape of both proteins and lipid bilayer were distinct in plain sight. Therefore, I was able to fit the model into the map manually. Is there a way to calculate the correlation coefficient between the model and map? Or, Could I fit the model into the low resolution map automatically?
>
> Sincerely,
> Si Hoon Park
>
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--

Skalidis Ioannis, MSc
Biochemistry & Cryo-EM Scientist
PhD Candidate
Kastritis Laboratory for Biomolecular Research
Cryo-Electron Microscopy & Computational Structural Biology

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