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Thanks all,
It would be very useful!
For fitting the model into the map, it looks like I need to merge the
multiple models into one PDB file.
I guess I have to get used to Chimera first:)

Best regards,
Si Hoon
ᐧ

On Tue, Jun 28, 2022 at 5:33 PM Ioannis Skalidis <[log in to unmask]>
wrote:

> Marino is right, ideally, to get a map-model correlation you would use
> Phenix' Cryo-EM validation suite, but I'm not sure at this resolution if
> it's that meaningful either.
>
> Cheers,
>
> Yiannis.
>
> On 28.06.22 10:21, Marino Jacopo (PSI) wrote:
> > Hi - you can do that in Chimera, for example. But the correlation number
> won't mean much.
> >
> > Cheers
> >
> > -----Original Message-----
> > From: Collaborative Computational Project in Electron cryo-Microscopy <
> [log in to unmask]> On Behalf Of Si Hoon Park
> > Sent: Tuesday, 28 June 2022 10:03
> > To: [log in to unmask]
> > Subject: [ccpem] Atomic model fitting in cryoET map
> >
> > Dear CCPEM community,
> > Thank you for all your efforts in the field of structural biology.
> > I would like to ask you about how to fit the model into a low resolution
> map.
> > I've got an electron density map of the protein oligomer on the lipid
> bilayer at ~25 A resolution.
> > Although the resolution was low, the shape of both proteins and lipid
> bilayer were distinct in plain sight. Therefore, I was able to fit the
> model into the map manually. Is there a way to calculate the correlation
> coefficient between the model and map? Or, Could I fit the model into the
> low resolution map automatically?
> >
> > Sincerely,
> > Si Hoon Park
> >
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> --
>
>
>
> Skalidis Ioannis, MSc
> Biochemistry & Cryo-EM Scientist
> PhD Candidate
> Kastritis Laboratory for Biomolecular Research
> Cryo-Electron Microscopy & Computational Structural Biology
>
> ________________________________________________
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