Thanks all, It would be very useful! For fitting the model into the map, it looks like I need to merge the multiple models into one PDB file. I guess I have to get used to Chimera first:) Best regards, Si Hoon ᐧ On Tue, Jun 28, 2022 at 5:33 PM Ioannis Skalidis <[log in to unmask]> wrote: > Marino is right, ideally, to get a map-model correlation you would use > Phenix' Cryo-EM validation suite, but I'm not sure at this resolution if > it's that meaningful either. > > Cheers, > > Yiannis. > > On 28.06.22 10:21, Marino Jacopo (PSI) wrote: > > Hi - you can do that in Chimera, for example. But the correlation number > won't mean much. > > > > Cheers > > > > -----Original Message----- > > From: Collaborative Computational Project in Electron cryo-Microscopy < > [log in to unmask]> On Behalf Of Si Hoon Park > > Sent: Tuesday, 28 June 2022 10:03 > > To: [log in to unmask] > > Subject: [ccpem] Atomic model fitting in cryoET map > > > > Dear CCPEM community, > > Thank you for all your efforts in the field of structural biology. > > I would like to ask you about how to fit the model into a low resolution > map. > > I've got an electron density map of the protein oligomer on the lipid > bilayer at ~25 A resolution. > > Although the resolution was low, the shape of both proteins and lipid > bilayer were distinct in plain sight. Therefore, I was able to fit the > model into the map manually. Is there a way to calculate the correlation > coefficient between the model and map? Or, Could I fit the model into the > low resolution map automatically? > > > > Sincerely, > > Si Hoon Park > > > > ######################################################################## > > > > To unsubscribe from the CCPEM list, click the following link: > > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCPEM&A=1 > > > > This message was issued to members of www.jiscmail.ac.uk/CCPEM, a > mailing list hosted by www.jiscmail.ac.uk, terms & conditions are > available at https://www.jiscmail.ac.uk/policyandsecurity/ > > > > ######################################################################## > > > > To unsubscribe from the CCPEM list, click the following link: > > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCPEM&A=1 > > > > This message was issued to members of www.jiscmail.ac.uk/CCPEM, a > mailing list hosted by www.jiscmail.ac.uk, terms & conditions are > available at https://www.jiscmail.ac.uk/policyandsecurity/ > > -- > > > > Skalidis Ioannis, MSc > Biochemistry & Cryo-EM Scientist > PhD Candidate > Kastritis Laboratory for Biomolecular Research > Cryo-Electron Microscopy & Computational Structural Biology > > ________________________________________________ > Martin-Luther-Universität Halle-Wittenberg > Biozentrum, Room A.2.21 > IWE ZIK HALOmem NWG III > "Kryo-Elektronenmikroskopie an Membranproteinkomplexen" > Weinbergweg 22, 06120 Halle > tel: +49 345 5524986 > web (Lab): https://blogs.urz.uni-halle.de/kastritislab/ > web (HALOmem): https://www.halomem.de/en/ > > ######################################################################## > > To unsubscribe from the CCPEM list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCPEM&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCPEM, a mailing > list hosted by www.jiscmail.ac.uk, terms & conditions are available at > https://www.jiscmail.ac.uk/policyandsecurity/ > ######################################################################## To unsubscribe from the CCPEM list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCPEM&A=1 This message was issued to members of www.jiscmail.ac.uk/CCPEM, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/