I am trying to autopick (relion-4.0-beta-2-commit-ce2e93) by using template matching:

++++ Executing new job on Wed Jun 22 12:03:05 2022
++++ with the following command(s):
`which relion_autopick_mpi` --i CtfFind/job009/micrographs_ctf.star --odir AutoP
ick/job070/ --pickname autopick --ref empty_from_2019_scaled.mrc --sym I2 --heal
pix_order 1 --invert --ctf --ang 30 --shrink 0 --lowpass 20 --angpix 0.97 --an
gpix_ref 0.97 --threshold 0.5 --min_distance 200 --max_stddev_noise 1.1 --partic
le_diameter 330 --pipeline_control AutoPick/job070/
++++
and experience a crash:

MPI noticed that process rank 2 with PID 0 on node BlaasLinux exited on signal 11 (Segmentation fault).

GNU nano 4.8 run.err Modified
#[BlaasLinux:2254462] *** Process received signal ***
[BlaasLinux:2254462] Signal: Segmentation fault (11)
[BlaasLinux:2254462] Signal code: Address not mapped (1)
[BlaasLinux:2254462] Failing at address: 0x28
[BlaasLinux:2254462] [ 0] /lib/x86_64-linux-gnu/libpthread.so.0(+0x143c0)[0x7f01f2e0a3c0]
[BlaasLinux:2254462] [ 1] /opt/relion-4.0/relion/build/bin/relion_autopick_mpi(_ZN10AutoPicker10initialiseEi+0x10fe)[0x564269a00e7e]
[BlaasLinux:2254462] [ 2] /opt/relion-4.0/relion/build/bin/relion_autopick_mpi(main+0x5d)[0x5642699d665d]
[BlaasLinux:2254462] [ 3] /lib/x86_64-linux-gnu/libc.so.6(__libc_start_main+0xf3)[0x7f01f28880b3]
[BlaasLinux:2254462] [ 4] /opt/relion-4.0/relion/build/bin/relion_autopick_mpi(_start+0x2e)[0x5642699da03e]
[BlaasLinux:2254462] *** End of error message ***
[BlaasLinux:2254461] *** Process received signal ***
[BlaasLinux:2254461] Signal: Segmentation fault (11)
[BlaasLinux:2254461] Signal code: Address not mapped (1)
[BlaasLinux:2254461] Failing at address: 0x28
[BlaasLinux:2254461] [ 0] /lib/x86_64-linux-gnu/libpthread.so.0(+0x143c0)[0x7f3fd0af43c0]
[BlaasLinux:2254461] [ 1] /opt/relion-4.0/relion/build/bin/relion_autopick_mpi(_ZN10AutoPicker10initialiseEi+0x10fe)[0x55fbc92e8e7e]
[BlaasLinux:2254461] [ 2] /opt/relion-4.0/relion/build/bin/relion_autopick_mpi(main+0x5d)[0x55fbc92be65d]
[BlaasLinux:2254461] [ 3] /lib/x86_64-linux-gnu/libc.so.6(__libc_start_main+0xf3)[0x7f3fd05720b3]
[BlaasLinux:2254461] [ 4] /opt/relion-4.0/relion/build/bin/relion_autopick_mpi(_start+0x2e)[0x55fbc92c203e]
[BlaasLinux:2254461] *** End of error message ***

Roughly the same error I get when using 2D references. However, Laplacian works just fine!

Sorry if it is just a stupid error from my side, thanks a lot for hints, best, Dieter