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Dear Layan, For higher-level analyses the data will already be in a standard space ( almost certainly MNI ) so the MNI standard mask should be fine - an exact fit isn’t critical here as it’s more important that no brain regions are excluded. Kind Regards Matthew -------------------------------- Dr Matthew Webster FMRIB Centre John Radcliffe Hospital University of Oxford On 20 Apr 2022, at 17:12, Layan Elfaki <[log in to unmask]<mailto:[log in to unmask]>> wrote: Dear FSL experts, I'm currently implementing TFCE analysis on seed-based first-level results through randomise, but I was curious about the -m portion of the command line argument. I understand it refers to the mask inputted, and I've been using a 2mm standard MNI mask as the input for this. I looked through the archives of the forum, but I'm not able to discern if this is correct or if I should be using a mask registered to the subjects' brains? Any help is appreciated Kind regards, Layan ________________________________ To unsubscribe from the FSL list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=FSL&A=1 ######################################################################## To unsubscribe from the FSL list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=FSL&A=1 This message was issued to members of www.jiscmail.ac.uk/FSL, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/