Hey Suchi,

you could firstly increase the binning to factor to 4-5. Also, it would help to shrink the mask radius. Most definitely keep the resolution limited to 8-10 Å!

Cheers

Schara
Dr. Schara Safarian

Max Planck Institut für Biophysik 
Abteilung für Molekulare Membranbiologie

Max Planck Institute of Biophysics 
Department of Molecular Membrane Biology 

Max-von-Laue-Straße 3
60438 Frankfurt am Main
Deutschland/Germany

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Am 01.06.2020 um 07:18 schrieb Suchi Goel <[log in to unmask]>:

Hi Everyone,

I am new to this list. Have some problem of background in 2D classification, that I can't get rid of for any parameter of regularization  (1.4-2.0) or resolution limit (-1, no limit and 7-12 A limit). 
Attached files are the output classes in Binning 2 and 1 respectively.
Could some one help who has this experience. 

Best Regards,
Suchi Goel

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