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We don't use the Siemens Immulite 2000 TSI assay in our lab (we use the BRAHMS Thermofisher TRAK assay (radioimmunoassay) so I can't comment on experience.

However, I can comment on the performance of the assay as we did a 3-way evaluation back in 2016 with one of our Endocrinologist Dr Albert Hsieh for this PhD. We compared the Siemens TSI against the RIA method and the Thyretain Thyroid Stimulating and Blocking Immunoglobulins functional bioassay.

The Siemens TSI had a really good sensitivity but unfortunately the lowest specificity. PPV was also the lowest of the 3 (0.91) and NPV (0.76).

Dr Hsieh presented this at the 2016 Endocrine Society of Australia/ Society of Reproductive Biology Annual Scientific Meeting.

We concluded that the Siemens TSI assay was not specific to the Stimulating immunoglobins as they claimed. It's another TRAB assay and this was supported by a few papers in the literature. Eg 2016 Oct;39(10):1159-65. doi: 10.1007/s40618-016-0478-9. Epub 2016 May 19.

Kind Regards

Julie Sherfan

Senior Hospital Scientist

Department of Chemical Pathology, Royal Prince Alfred Hospital


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From: Clinical biochemistry discussion list <[log in to unmask]> on behalf of DAWNAY, Anne (BARTS HEALTH NHS TRUST) <[log in to unmask]>
Sent: Saturday, 7 March 2020 02:17
To: [log in to unmask]
Subject: Immulite 2000 TSI assay

A colleague is asking if anyone has experience of using Siemens Immulite 2000 TSI assay which has appeared on the market recently and can detect quantitatively and specifically the thyroid-stimulating immunoglobulins only.

Best wishes

Anne

Dr Anne Dawnay PhD FRCPath

Consultant Clinical Biochemist

Tel 020 7377 7000 ext 61030

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