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Hi, For t-contrasts, the test is for a positive relationship ( although obviously you can flip the sign of the contrast to look the other way ). Hope this helps, Kind Regards Matthew -------------------------------- Dr Matthew Webster FMRIB Centre John Radcliffe Hospital University of Oxford On 8 Jan 2020, at 17:19, Erin Moe <[log in to unmask]<mailto:[log in to unmask]>> wrote: Thanks for the quick reply! That's what I assumed, however, does this denote a statistically significant positive relationship or negative and the effect of the covariate on the FA. Would I need to extract the FA from those regions and do further statistical analysis to determine the exact relationship? Thanks! Erin ######################################################################## To unsubscribe from the FSL list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=FSL&A=1 ######################################################################## To unsubscribe from the FSL list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=FSL&A=1