Hi Almu,

this is most often the case, when your beam center is not assigned correctly. Try to find out the beam center:

https://strucbio.biologie.uni-konstanz.de/xdswiki/index.php/Finding_out_ORGX_ORGY

Another possibilty is that the distance that you get from the header/machine is not very accurate. You can try to slightly adjust your distance and see, whether this improves your indexing (e.g. instead of 50 start from 52).

In XDS.INP you can enable distance refinement (as well as beam center refinement) throughout all steps. Just remove the "!" in "Refine"

Hope this helps,

Best wishes,

Raphael

On 29.11.19 12:48, Almudena Ponce Salvatierra wrote:

Dear all,

I have some data sets that don't want to be processed :p

In one of them, when I look at IDXREF.LP I see virtually none of the found spots were indexed and the reason is that they are "too far from the expected position". The spots are smeary and elongated, so not the prettiest.

I have managed to process so far only one data set with decent statistics from another crystal harvested from the same drop, where the diffraction spots look better.

I am trying to find in the xds wiki the keyword I should fine tune in order to make those spots indexable.

Could you help me please?

Thank you very much in advance.

Best wishes,

Almu

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-- 
Dr. Raphael Gasper-Schönenbrücher
Crystallography and Biophysics Facility
Max-Planck-Institute of Molecular Physiology
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Otto-Hahn-Str. 11
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