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Hi Raphael, Thank you very much. I tried yesterday with different origins and I have changed the REFINE(IDXREF) keyword several times as to refine BEAM, POSITION, DISTANCE... I have to keep trying because fo far I didn't succeed. I will get back to you once I find what the problem is. Thank you once again! Best, Almudena El vie., 29 nov. 2019 a las 12:56, R. Gasper-Schönenbrücher (< [log in to unmask]>) escribió: > Hi Almu, > > this is most often the case, when your beam center is not assigned > correctly. Try to find out the beam center: > > > https://strucbio.biologie.uni-konstanz.de/xdswiki/index.php/Finding_out_ORGX_ORGY > Another possibilty is that the distance that you get from the > header/machine is not very accurate. You can try to slightly adjust your > distance and see, whether this improves your indexing (e.g. instead of 50 > start from 52). > > In XDS.INP you can enable distance refinement (as well as beam center > refinement) throughout all steps. Just remove the "!" in "Refine" > > Hope this helps, > > Best wishes, > > Raphael > > > On 29.11.19 12:48, Almudena Ponce Salvatierra wrote: > > Dear all, > > I have some data sets that don't want to be processed :p > > In one of them, when I look at IDXREF.LP I see virtually none of the found > spots were indexed and the reason is that they are "too far from the > expected position". The spots are smeary and elongated, so not the > prettiest. > > I have managed to process so far only one data set with decent statistics > from another crystal harvested from the same drop, where the diffraction > spots look better. > > I am trying to find in the xds wiki the keyword I should fine tune in > order to make those spots indexable. > > Could you help me please? > > Thank you very much in advance. > > Best wishes, > > Almu > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > > -- > Dr. Raphael Gasper-Schönenbrücher > Crystallography and Biophysics Facility > Max-Planck-Institute of Molecular Physiology > Room F0.92 > Otto-Hahn-Str. 11 > 44227 Dortmund, Germany > Tel +49 (0)231-133 2729 > > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1