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Thank you all, for your replies! Some of you suggested that there could be reflections at the corners of the detector, which are 'cutted by the edges'. This also came to my mind, but the data was collected until 1.7A at the edges. I will take the advice to refine against the low and the high resolution cutted datasets and compare them. (For high resolution limit refinement I got a R-work of 0.2098 and R-free of 0.2655 after a few refinement cycles with phenix using isotropic parameters) Thanks again for your help. Best regards, Matthias Oebbeke Zitat von "Winter, Graeme (DLSLtd,RAL,LSCI)" <[log in to unmask]>: > The data are > 90% complete at low resolution, where the > completeness is more important, so this is probably fine - you would > probably benefit from better detector placement in the future as > the data are good to the edge. > > Was the detector offset or just too far away? 120° for an oP lattice > should be relatively complete - as appears to be the case for low > angle data here. > > The reflections you have measured are almost certainly a better > estimate of F^2 than 0, which is what you are saying if you do throw > them away :-) > > Best wishes Graeme > >> On 25 Nov 2019, at 13:11, Matthias Oebbeke >> <[log in to unmask]> wrote: >> >> Dear ccp4 Bulletin Board, >> >> I collected a dataset at a synchrotron beamline and got the >> statistics (CORRECT.LP) after processing (using xds) shown in the >> attached pdf-file. >> >> Do you think this dataset is usable, due to its low completeness? >> As you can see in the attached file the completeness is just 50% in >> the highest resolution shell, whereas the I over Sigma is above 2 >> and also the CC 1/2 (80%) and the R factors (36.8%) have reasonable >> values. Furthermore the overall statistic are good regarding R >> factor, CC 1/2 and I over Sigma. The only problem seems to be the >> completeness. If I would set the cut-off at a lower resolution to >> get good completeness, I would throw away nearly half of my >> reflections. >> >> I'm happy to here your opinion. In Addition to that: The space >> group is orthorhombic and the dataset was collected over 120° using >> fine slicing (0.1°). >> >> >> Best regards, >> >> Matthias Oebbeke >> >> >> -- >> Matthias Oebbeke, M.Sc. >> Research Group of Professor Dr. G. Klebe >> Institute of Pharmaceutical Chemistry >> Philipps-University Marburg >> Marbacher Weg 6, 35032 Marburg, Germany >> Phone: +49-6421-28-21392 >> Mail: [log in to unmask] >> www.agklebe.de >> >> ######################################################################## >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 >> <CORRECT_LP.pdf> > > > -- > This e-mail and any attachments may contain confidential, copyright > and or privileged material, and are for the use of the intended > addressee only. If you are not the intended addressee or an > authorised recipient of the addressee please notify us of receipt by > returning the e-mail and do not use, copy, retain, distribute or > disclose the information in or attached to the e-mail. > Any opinions expressed within this e-mail are those of the > individual and not necessarily of Diamond Light Source Ltd. > Diamond Light Source Ltd. cannot guarantee that this e-mail or any > attachments are free from viruses and we cannot accept liability for > any damage which you may sustain as a result of software viruses > which may be transmitted in or with the message. > Diamond Light Source Limited (company no. 4375679). Registered in > England and Wales with its registered office at Diamond House, > Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 > 0DE, United Kingdom -- Matthias Oebbeke, M.Sc. Research Group of Professor Dr. G. Klebe Institute of Pharmaceutical Chemistry Philipps-University Marburg Marbacher Weg 6, 35032 Marburg, Germany Phone: +49-6421-28-21392 Mail: [log in to unmask] www.agklebe.de ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1