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Dear Gloria, This is the basic protocol we follow. Hope it helps Arnaud Gautier and Marlon J. Hinner (eds.), Site-Specifi c Protein Labeling: Methods and Protocols, Methods in Molecular Biology, vol. 1266, DOI 10.1007/978-1-4939-2272-7_12, © Springer Science+Business Media New York 2015 Linda ________________________________ From: CCP4 bulletin board <[log in to unmask]> on behalf of Gloria Borgstahl <[log in to unmask]> Sent: Monday, October 21, 2019 12:22 PM To: [log in to unmask] <[log in to unmask]> Subject: [ccp4bb] E. coli BirA biotin ligase expression/purification ATTENTION: This email originated from a sender outside of MCW. Use caution when clicking on links or opening attachments. ________________________________ Dear friends in crystallography, I know this seems unrelated, but it really isn't ... please forgive me. We are trying to use the Avitag/BirA system to specifically biotinylate target proteins in an economical manner. Are any of you purifying the BirA enzyme in your lab for biotinylation and if so can you help me figure out the right plasmid/purification protocol? Please let me know. Thank you. Gloria ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFaQ&c=aFamLAsxMIDYjNglYHTMV0iqFn3z4pVFYPQkjgspw4Y&r=iHKIgRWrjiS1dpoNu_8JEA&m=G-X7Orq-6E-4lIXTlihyWYVCq3HqYZlxVwVvvz-ZdO0&s=0NRMvIxzXZM_8e_Y3kg7SIMwmj9Y2sR4ol8QPSWeXto&e=> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1