Hi,
Can you expand on your steps? Siena should be used on T1 images, the halfway transforms can then be combined each timepoint’s DTI-> T1 transforms to obtain DTI images in halfway-space ( that can be used e.g. to form a template ).

Also can you confirm your tbss pipeline - are you ( effectively ) inputting all the subjects to tbss_2_reg, but using the -t template option.

Kind Regards
Matthew
--------------------------------
Dr Matthew Webster
FMRIB Centre 
John Radcliffe Hospital
University of Oxford

On 17 Sep 2019, at 10:00, Mónica Emch <[log in to unmask]> wrote:

Dear FSL community,

to avoid removal of within-subject longitudinal differences, we created a nonbiased subject-specific template, which was obtained with SIENA (fsl 6.0.0). I got A_halfwayto_B.nii.gz and B_halfwayto_A.nii.gz with SIENA command (siena dtifit_subject1_1_FA.nii.gz dtifit_subject1_2_FA.nii.gz -B "-f 0.2" -d -t2).

Afterwards, with fslmaths I created an average of these two images and these base FA templates are the one that I used for the input in TBSS (fslmaths A_halfwayto_B.nii.gz -add B_halfwayto_A.nii.gz -div 2 halfway_template.nii.gz). Once the TBSS is done, I do not get a full mean FA skeleton, but only the central part.

Did someone has this problem before?

Thank you in advance,

Cheers,

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